An automated programmable platform enabling multiplex dynamic stimuli delivery and cellular response monitoring for high-throughput suspension single-cell signaling studies† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4lc01070a Click here for additional data file.
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چکیده
Figure S1. The stimulus profile visualized using FITC-BSA shows similar pattern to that of fluorescein, although FITC-BSA pattern are more dispersed compared to that of fluorescein. Profiles were generated by alternatively delivering FITC-BSA solution and PBS at 50 mHz driven by (A) 1psi, (B) 2psi, (C) 3psi and (D) 4psi. Heatmaps of spatial (Y axis) and temporal (X axis) show fluorescent intensity (color bar) in single observation chamber.
منابع مشابه
An automated programmable platform enabling multiplex dynamic stimuli delivery and cellular response monitoring for high-throughput suspension single-cell signaling studies.
Cell signaling events are orchestrated by dynamic external biochemical cues. By rapidly perturbing cells with dynamic inputs and examining the output from these systems, one could study the structure and dynamic properties of a cellular signaling network. Conventional experimental techniques limit the implementation of these systematic approaches due to the lack of sophistication in manipulatin...
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